A number of guidelines or rubrics govern which icons are on which parts of the iconostasis, although there is some room for variation. There are also guidelines for who should enter or leave the altar by which door. These guidelines were developed over the course of many centuries, with both theologically symbolic and practical reasons for them.
Though they vary in size, shape and number of icons, the following is a basic layout of an icon screen which one might find in typical parish church.
- An icon of the Theotokos with the Lord. This indicates the beginning of the end of time, the time of our salvation.
- An icon of The Lord, usually as All-ruler (Pantocrator), the just judge of all our works. This indicates the end of all time, the awesome day of judgment.
- Icon of Saint John, the Prophet, Forerunner, and Baptizer of the Lord.
- Icon of the patron of the temple, or of its patronal feast.
- The Holy Doors (or the Royal Doors). These usually are a diptych of the Annunciation. Sometimes they may also have the icons of the four evangelists. This entrance is reserved for the use of the bishop, and the priest when he is carrying either the Gospel book or the chalice containing the holy Eucharist. In other words, it is reserved liturgically for the use of Christ as master and Lord.
- North door (the north and south doors are often called "deacon's doors"). This will often depict an archangel, almost always St. Michael. This door is liturgically the exit from the altar, often interpreted mystically as heaven. Thus, St. Michael guards the door to heaven. This icon is also sometimes a deacon, usually St. Stephen the Protomartyr.
- South door. This door is the liturgical entrance to the altar, interpreted mystically as heaven. The archangel on this door is St. Gabriel, whose announcement to the Theotokos marks the beginning of the Incarnation, which is our entrance to the heavenly realm. If a deacon is depicted, it is usually St. Philip or St. Lawrence.
- These icons (when present) are usually saints especially near to a parish or nation, such as Ss. Nicholas of Myra, George the Trophy-bearer, Demetrius the Myrrh-streaming, Sergius of Radonezh, Andrew the First-called, Herman of Alaska, or Seraphim of Sarov.
- This is usually the icon of the Mystical Supper, the last supper our Lord ate with his friends and wherein he instituted the Eucharist.
If there is a second tier, it will usually contain icons of the Twelve Great Feasts. Other tiers will depict the patriarchs, prophets and apostles.